Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Naive T cells

NA

NA

Sample

source_name

Naïve CD4+ T cells from spleen

strain

C57BL/6

tissue

spleen

cell type

naive CD4+ T cells

genotype

Stat3-fl/fl; Il7r-Cre

polarization

Th17

batch

B4

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Cells harvest was followed by a transposition reaction and purification (Buenrostro et al. Curr Protoc Mol Biol. 2015) Prepared as in Buenrostro et al. Curr Protoc Mol Biol. 2015

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

17624190

Reads aligned (%)

96.0

Duplicates removed (%)

30.5

Number of peaks

8082 (qval < 1E-05)

mm9

Number of total reads

17624190

Reads aligned (%)

95.9

Duplicates removed (%)

30.7

Number of peaks

8093 (qval < 1E-05)