ChIP-Atlas: SRX3990065

Antigen

Cell type Class: Blood
Cell type: Macrophages
MeSH Description: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: ATAC-seq was performed using a modified protocol based on the technique published by Buenrostro et al. Briefly, sort-purified cell populations were washed in PBS and lysed with lysis buffer (1 M Tris•Cl, pH 7.4, 5 M NaCl, 1 M MgCl2; 10% NP-40 in nuclease-free H2O) and gentle agitation prior to treatment with Tn5 transposase. DNA was purified (Qiagen MinElute Reaction Cleanup Kit), and amplified with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs) using barcoded primers. DNA library was purified with AMPure XP beads, and library quality was assayed via Qubit and Agilent High Sensitivity DNA Bioanalysis chip. Libraries were sequenced via 40 bp paired-end sequencing using the Illumina NextSeq 500 platform.