Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver tissue, DKO, 3 months, H3K27me3 ChIP

strain/background

129SV::FVB/N::C57BL/6

genotype/variation

Ezh1-/-Ezh2fl/flAlb-Cre

tissue

liver

age

3 months

chip antibody

H3K27me3 (in-house antibody by Active Motif, Carlsbad, CA)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-sequencing was initially performed and processed by Active Motif, Inc. (Carlsbad, CA) on mouse liver tissues. Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

57591754

Reads aligned (%)

97.5

Duplicates removed (%)

55.4

Number of peaks

439 (qval < 1E-05)

mm9

Number of total reads

57591754

Reads aligned (%)

97.4

Duplicates removed (%)

55.5

Number of peaks

428 (qval < 1E-05)