Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Neural
Cell type
T98G
Primary Tissue
Brain
Tissue Diagnosis
Glioblastoma

Attributes by original data submitter

Sample

source_name
T98G
cell line
T98G
cell type
Glioblastoma multiforme cell line
chip antibody
H3K27me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Antibodies used were H3K4me3 (EMD Millipore, 07-473) and H3K27me3 (Millipore 07-449). Libraries were prepared according to the New England Biolabs NEBNext library prep kit (E6240L). Ampure XP beads (A63881) were used for size-selection and clean-up steps.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
30691059
Reads aligned (%)
96.0
Duplicates removed (%)
4.4
Number of peaks
852 (qval < 1E-05)

hg19

Number of total reads
30691059
Reads aligned (%)
95.5
Duplicates removed (%)
4.6
Number of peaks
729 (qval < 1E-05)

Base call quality data from DBCLS SRA