Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver cells

strain

C57BL/6

chip antibody

H3K27me3

age

3 month

genotype/variation

wild type

tissue

normal liver

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with different antibodies. NEBNext® Ultra™ DNA Library Prep Kit (NEB, UK)

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

49632176

Reads aligned (%)

99.5

Duplicates removed (%)

5.9

Number of peaks

363 (qval < 1E-05)

mm9

Number of total reads

49632176

Reads aligned (%)

99.4

Duplicates removed (%)

6.0

Number of peaks

355 (qval < 1E-05)