Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Liver
Cell type
Liver
MeSH Description
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Attributes by original data submitter

Sample

source_name
Liver cells
strain
C57BL/6
chip antibody
H3K27me3
age
18 month
genotype/variation
HBx-TG
tissue
tumor

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with different antibodies. NEBNext® Ultra™ DNA Library Prep Kit (NEB, UK)

Sequencing Platform

instrument_model
Illumina HiSeq 1500

mm10

Number of total reads
48803128
Reads aligned (%)
99.5
Duplicates removed (%)
6.5
Number of peaks
321 (qval < 1E-05)

mm9

Number of total reads
48803128
Reads aligned (%)
99.3
Duplicates removed (%)
6.5
Number of peaks
316 (qval < 1E-05)

Base call quality data from DBCLS SRA