Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Liver

Cell type

Hepatocytes

MeSH Description

The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.

Sample

source_name

hepatocytes

age

8 weeks old

strain

DBA/2J

ChIP

Input DNA

antibody

NA

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were isolated from cells using hypotonic lysis. Chromatin was fragmented by Mnase digestion. After high speed spin soluble chromatin was removed and used for ChIP Libraries were prepared for sequencing using Bioo Scientific's NEXTflex ChIP-Seq Kit (protocol version V11.11) without size selection for all germ cell data or the KAPA hyper kit (Kapa Biosystems) for mESC, cardiomyocytes, and hepatocytes.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

44985640

Reads aligned (%)

97.8

Duplicates removed (%)

10.7

Number of peaks

249 (qval < 1E-05)

mm9

Number of total reads

44985640

Reads aligned (%)

97.7

Duplicates removed (%)

10.7

Number of peaks

202 (qval < 1E-05)