Nuclei were isolated from cells using hypotonic lysis. Chromatin was fragmented by Mnase digestion. After high speed spin soluble chromatin was removed and used for ChIP Libraries were prepared for sequencing using Bioo Scientific's NEXTflex ChIP-Seq Kit (protocol version V11.11) without size selection for all germ cell data or the KAPA hyper kit (Kapa Biosystems) for mESC, cardiomyocytes, and hepatocytes.