Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived mesenchymal stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mesenchymal stem cell
cell type
ES-derived mesenchymal stem cell
genotype
DR8dex2
passage
p3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was isolated using phenol-chloroform-isoamylalcohol extraction and ethanol precipitation. The H3K9me3 enriched fragments were contructed into libararies via KAPA Hyper Prep Kits with PCR Library Amplification/Illumina series(KK8504) following the manuals.

Sequencing Platform

instrument_model
HiSeq X Ten

hg38

Number of total reads
12854760
Reads aligned (%)
87.6
Duplicates removed (%)
10.3
Number of peaks
1040 (qval < 1E-05)

hg19

Number of total reads
12854760
Reads aligned (%)
84.3
Duplicates removed (%)
10.9
Number of peaks
294 (qval < 1E-05)

Base call quality data from DBCLS SRA