GSM1291199: Tal1 ChIP With TSA Treatment; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TAL1
Cell type
Cell type Class
Cardiovascular
Cell type
Primary endothelial cells
NA
NA
Attributes by original data submitter
Sample
source_name
Human primary endothelial colony-forming cells
source
ECFCs derived from human umbilical cord blood
antibody
anti-TAL1 (C-21, Santa-Cruz)
treatment
100 ng/ml final TSA
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Binding of TAL1 transcription factor was measured using a ChIP protocol as previously described (Palii, 2011). Chromatin from 0.5x10^8 cells was fragmented to a size range of 100-300 bases with a Bioruptor (Diagenode) at 4°C. Solubilized chromatin was immunoprecipitated with an antibody against TAL1 (C-21, Santa-Cruz) or an isotype IgG control. Antibody-chromatin complexes were pulled-down using Dynabeads-Protein G, washed and eluted. After cross-link reversal and proteinase K treatment, immunoprecipitated DNA was extracted with phenol-chloroform. ChIPed DNA was amplified using the Illumina protocol with modifications described in (Palii, 2011).