GSM3096433: TAMR 4OHT H3K27Ac KC171 Rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
TAMR
cell line
TAMR
treatment
4OHT
treatment time
45min
factor
H3K27Ac
chip antibody
Diagenode, C15410196
replicate
Rep2
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell pellets were thawed on ice, and then resuspended in Lysis Buffer containing 1% SDS, 0.25mM EDTA, 2.5mM Tris-HCl, pH 8.0, protease inhibitor cocktail. Cell lysates were sonicated using the Covaris. KAPA HTP