Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

TAMR

cell line

TAMR

treatment

4OHT

treatment time

45min

factor

H3K27Ac

chip antibody

Diagenode, C15410196

replicate

Rep1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell pellets were thawed on ice, and then resuspended in Lysis Buffer containing 1% SDS, 0.25mM EDTA, 2.5mM Tris-HCl, pH 8.0, protease inhibitor cocktail. Cell lysates were sonicated using the Covaris. KAPA HTP

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

18096475

Reads aligned (%)

98.6

Duplicates removed (%)

10.2

Number of peaks

28051 (qval < 1E-05)

hg19

Number of total reads

18096475

Reads aligned (%)

98.2

Duplicates removed (%)

10.7

Number of peaks

27973 (qval < 1E-05)