GSM3096406: MesoT ChIP K27ac; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC WA09
NA
NA
Attributes by original data submitter
Sample
source_name
Human Embryonic Stem Cells
cell line
WA09
passages
47-60
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were collected using collagenase type IV (MesoT) and/or TrypLE Select (MesoT, MesoT FCS, MLC, smooth muscle). The outer mesothelial layer of mouse organs were digested by exposing intact organs to a dissociation buffer containing 1 mg/ml collagenase IV and 0.05% trypsin. All cells were homogenized with Omega Homogenizer Columns (HCR003). Total RNA were extracted using Trizol and purified with RNeasy mini kit (Qiagen) according to manufacture's protocol. Samples with an RNA integrity number (RIN) above 0.9 were selected and sent out for standard RNA-Seq application, including poly A enrichment, adaptor ligation, bar-coding, library amplification, etc (Hudsonalpha, AL, US) and Georgia Genomics Facility (University of Georiga, US). RNA-Seq, 50bp and 75bp pair-end, at least 25 million reads per sample; ChIP-seq, 50bp single-end, 25 millioms reads per sample