Antigen

Antigen Class

TFs and others

Antigen

Bhlhe40

Cell type

Cell type Class

Blood

Cell type

Bone Marrow Cells

MeSH Description

Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Sample

source_name

GM-CSF-cultured bone marrow derived cells

cell type

Macrophage, DC, granulocyte mix

bhlhe40

WT

strain

C57BL/6

chip antibody

anti-Bhlhe40 (Novus Biologicals NB100-1800, Lot A)

treatment

10ug/mL heat-killed Mtb for 4 hours

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Stimulated cells were fixed for 10 minutes at room temperature with 1% paraformaldehyde with shaking. Cross-linked chromatin was fragmented by sonication and then immunoprecipitated with polyclonal rabbit anti-Bhlhe40 antibody (Novus Biologicals NB100-1800, Lot A or Lot C1). After immunoprecipitation, DNA was purified by the GenElute PCR clean-up kit (Sigma). Standard ChIP library prep for Illumina sequencing

Sequencing Platform

instrument_model

Illumina HiSeq 3000

mm10

Number of total reads

37658206

Reads aligned (%)

98.5

Duplicates removed (%)

39.1

Number of peaks

354 (qval < 1E-05)

mm9

Number of total reads

37658206

Reads aligned (%)

98.3

Duplicates removed (%)

39.1

Number of peaks

326 (qval < 1E-05)