Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

EOL-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

EOL1 (AML)

cell type

AML

cell line

EOL1

chip antibody

H3K4me3

antibody manufacturer

Millipore

antibody catalog number

05745R

antibody lot number

2326998

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed using standard protocols (Millipore, Billerica, MA). Specifically, cells were fixed in 1% formaldehyde (Sigma Aldrich, F8775) for 10min at 37C and quenched with 125mM glycine for 5min at 37C. After washing, nuclei were sonicated using Covaris Sonicator, and the supernatant was used for immunoprecipitation with the indicated antibody. ChIP-sequencing libraries were prepared with the Rubicon Thruplex DNAseq prep kit using standard protocols.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

50558228

Reads aligned (%)

97.7

Duplicates removed (%)

49.3

Number of peaks

24404 (qval < 1E-05)

hg19

Number of total reads

50558228

Reads aligned (%)

97.6

Duplicates removed (%)

49.5

Number of peaks

24311 (qval < 1E-05)