Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SMARCA4

Cell type

Cell type Class
Blood
Cell type
EOL-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
EOL1 (AML)
cell type
AML
cell line
EOL1
chip antibody
SMARCA4
antibody manufacturer
Abcam
antibody catalog number
EPNCIR111A
antibody lot number
GR150844-12

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed using standard protocols (Millipore, Billerica, MA). Specifically, cells were fixed in 1% formaldehyde (Sigma Aldrich, F8775) for 10min at 37C and quenched with 125mM glycine for 5min at 37C. After washing, nuclei were sonicated using Covaris Sonicator, and the supernatant was used for immunoprecipitation with the indicated antibody. ChIP-sequencing libraries were prepared with the Rubicon Thruplex DNAseq prep kit using standard protocols.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
42341658
Reads aligned (%)
81.3
Duplicates removed (%)
14.7
Number of peaks
88316 (qval < 1E-05)

hg38

Number of total reads
42341658
Reads aligned (%)
82.4
Duplicates removed (%)
14.0
Number of peaks
88703 (qval < 1E-05)

Base call quality data from DBCLS SRA