Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
BT-549
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
BT549 cells
cell line
BT549
cell type
ERalpha-negtive breast cancer cells
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei, IgG, Fra-1 and DDX5 complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
46049473
Reads aligned (%)
99.2
Duplicates removed (%)
6.1
Number of peaks
1171 (qval < 1E-05)

hg19

Number of total reads
46049473
Reads aligned (%)
98.4
Duplicates removed (%)
7.4
Number of peaks
1217 (qval < 1E-05)

Base call quality data from DBCLS SRA