Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Breast

Cell type

BT-549

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

BT549 cells

cell line

BT549

cell type

ERalpha-negtive breast cancer cells

chip antibody

normal rabbit IgG (sc-2027, Santa Cruz)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei, IgG, Fra-1 and DDX5 complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

45114278

Reads aligned (%)

97.8

Duplicates removed (%)

16.2

Number of peaks

1527 (qval < 1E-05)

hg19

Number of total reads

45114278

Reads aligned (%)

96.9

Duplicates removed (%)

18.0

Number of peaks

1508 (qval < 1E-05)