Total RNA was collected using Qiagen's Rneasy Mini Kit (74106) and assessed for RNA integrity number >9.5. For ChIP-seq, cells were fixed, quenched, sonicated to ~250 bp in 1% SDS lysis buffer, and incubated with Protein A Dynabeads (Novex 10008) and 5 µg of the indicated antibody. 2% pre-cleared chromatin prior to primary antibody addition was kept as input DNA. After wash, treatment with RNAse A and Proteinase K, DNA was eluted and purified with Qiagen's PCR purification kit (28106).For ATACSeq, cell nuclei were prepared from 5x104 cells and incubated with 2.5 μL transposase (Illumina) in a 50 μL reaction for 30 minutes at 37°C. For RNASeq, library construction was performed using TruSeq RNA Sample Prep Kit v2 (Illumina) following manufacturer's instructions. For ChIPSeq, DNA quality was checked using Agilent Technologies 2200 TapeStation and quantified using PicoGreen. Library construction was performed using the NEBNext ChIP-seq Library Prep Master Mix Set for Illumina according to manufacturer's conditions. Samples were pooled and submitted to New York Genome Center for SE50 sequencing. For ATACSeq, following purification of transposase-fragmented DNA, the library was amplified by PCR and subjected to PE50 high-throughput sequencing.