Human hematopoietic stem cells CD34+ freshly purified
5 days of HCMV infection
Sequenced DNA Library
For Chipseq CD34+ infected cells were crosslinked by 1% formaldehyde and sonicated, and chromatin was immunoprecipitated by using an affinity-purified rabbit polyclonal antibody raised against KAP1 aa 20–418, RBCC (F. R. Santoni de Sio et al.,. Blood 119, 4675 (2012)). Detailed protocols for ChIP are described in D. C. Schultz,. Genes Dev 15, 428 (2001). Reverse cross-linking was performed overnight in the presence of proteinase K and RNase, followed by DNA extraction using the MinElute PCR purification kit (Quiagen) according to manufacturer protocol. The library was generated from 10 ng total DNA using the TruSeq Illumina Preparation Kit.