Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Adult

Cell type

Testis

NA

NA

Sample

source_name

fruitfly testis nos>shRNA E(z)

tissue

testis specifically expressing E(z) shmiR RNAi in germ cells

chip antibody

none

sample type

input DNA

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

4% formaldehyde fixed testes were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. libraries are prepared following G. Wei, G. Hu, K. Cui, K. Zhao, Methods Enzymol 513, 297 (2012).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

8923441

Reads aligned (%)

90.4

Duplicates removed (%)

78.2

Number of peaks

380 (qval < 1E-05)

dm3

Number of total reads

8923441

Reads aligned (%)

90.6

Duplicates removed (%)

76.7

Number of peaks

512 (qval < 1E-05)