Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Adult
Cell type
Testis
NA
NA

Attributes by original data submitter

Sample

source_name
fruitfly testis nos>shRNA E(z)
tissue
testis specifically expressing E(z) shmiR RNAi in germ cells
chip antibody
H3K27me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
4% formaldehyde fixed testes were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. libraries are prepared following G. Wei, G. Hu, K. Cui, K. Zhao, Methods Enzymol 513, 297 (2012).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
13121939
Reads aligned (%)
27.0
Duplicates removed (%)
62.0
Number of peaks
161 (qval < 1E-05)

dm3

Number of total reads
13121939
Reads aligned (%)
27.1
Duplicates removed (%)
60.8
Number of peaks
240 (qval < 1E-05)

Base call quality data from DBCLS SRA