Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Gonad

Cell type

Oocytes

MeSH Description

Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).

Sample

source_name

oocyte

tissue

oocyte

developmental stage

FGO

chip antibody

H3K27me3 (Diagenode,Cat.C15410069)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The DNA extraction follows STAR ChIP-seq protocol The STAR ChIP-seq libraries were generated following the protocol as described previously(Zhang et al., 2016).

Sequencing Platform

instrument_model

HiSeq X Ten

mm10

Number of total reads

34811593

Reads aligned (%)

16.8

Duplicates removed (%)

46.6

Number of peaks

54 (qval < 1E-05)

mm9

Number of total reads

34811593

Reads aligned (%)

16.8

Duplicates removed (%)

46.4

Number of peaks

36 (qval < 1E-05)