Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
HAFTL
NA
NA

Attributes by original data submitter

Sample

source_name
Haftl pre-B cells
cell type
haftl derived C10 cells
time after induction
48 hours
chip antibody
H3K27Ac (ab4729, Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed as described previously (van Oevelen et al, 2008, PMID:20956564). Libraries were prepared according to manufacturer’s instructions with some modifications (Asp et al, 2011, PMID: 21551099). All libraries were sequenced on the Illumina 2AAIIx sequencer

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
28149460
Reads aligned (%)
85.3
Duplicates removed (%)
12.1
Number of peaks
40366 (qval < 1E-05)

mm9

Number of total reads
28149460
Reads aligned (%)
85.2
Duplicates removed (%)
12.2
Number of peaks
40413 (qval < 1E-05)

Base call quality data from DBCLS SRA