Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

Lin- BM progenitors (HSPCs)

strain

C57BL/6

genotype

EZH2 WT

chip antibody

H3K27Ac (Abcam, ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lineage negative bone marrow cells were used. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibodies. ChIP-Seq libraries were prepared for sequencing using a TruSeq ChIP Sample Prep Kit (Illumina) and standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

96400648

Reads aligned (%)

95.3

Duplicates removed (%)

14.3

Number of peaks

29460 (qval < 1E-05)

mm9

Number of total reads

96400648

Reads aligned (%)

95.3

Duplicates removed (%)

14.4

Number of peaks

29448 (qval < 1E-05)