Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Liver

Cell type

SNU-398

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

Hepatocarcinoma cell

cell line

SNU398

cell type

Hepatocarcinoma

chip antibody

H3K27Ac (Abcam, cat# Ab4729, Lot GR232896-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell were fixed with 1% formaldehyde and insoluble chromatin fraction were isolated. Chromatin were sonicated to 100 to 500 base pair and protein-DNA complexes were pulled down by antbodies Libraries were prepared using ThruPLEX DNA-seq kit (R400428) according to manufacturer's instruction. Constructed libraries were PAGE purified and only libraries with 250 to 500bp were used for sequencing

Sequencing Platform

instrument_model

Illumina MiniSeq

hg38

Number of total reads

29420647

Reads aligned (%)

98.7

Duplicates removed (%)

3.5

Number of peaks

23258 (qval < 1E-05)

hg19

Number of total reads

29420647

Reads aligned (%)

98.3

Duplicates removed (%)

3.5

Number of peaks

22948 (qval < 1E-05)