Antigen

Antigen Class

TFs and others

Antigen

SALL4

Cell type

Cell type Class

Liver

Cell type

SNU-398

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

Hepatocarcinoma cell

cell line

SNU398

cell type

Hepatocarcinoma

chip antibody

SALL4 (Cell Signaling Technolog, cat# 8459BF, Lot 2)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell were fixed with 1% formaldehyde and insoluble chromatin fraction were isolated. Chromatin were sonicated to 100 to 500 base pair and protein-DNA complexes were pulled down by antbodies Libraries were prepared using ThruPLEX DNA-seq kit (R400428) according to manufacturer's instruction. Constructed libraries were PAGE purified and only libraries with 250 to 500bp were used for sequencing

Sequencing Platform

instrument_model

Illumina MiniSeq

hg38

Number of total reads

37534587

Reads aligned (%)

98.6

Duplicates removed (%)

5.3

Number of peaks

3442 (qval < 1E-05)

hg19

Number of total reads

37534587

Reads aligned (%)

97.8

Duplicates removed (%)

5.6

Number of peaks

2722 (qval < 1E-05)