Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Embryo

Cell type

Blastocyst Inner Cell Mass

MeSH Description

The cluster of cells inside a blastocyst. These cells give rise to the embryonic disc and eventual embryo proper. They are pluripotent EMBRYONIC STEM CELLS capable of yielding many but not all cell types in a developing organism.

Sample

source_name

human embryo

development stage

ICM

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

After removing the zona pellucida, embryonic cells were washed with PBS and incubated in lysis buffer. ATAC-Seq libraries were prepared using ATAC-seq developed by Buenrostro et al. with slightly modification

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

276293341

Reads aligned (%)

25.1

Duplicates removed (%)

79.2

Number of peaks

336 (qval < 1E-05)

hg19

Number of total reads

276293341

Reads aligned (%)

24.9

Duplicates removed (%)

79.6

Number of peaks

230 (qval < 1E-05)