GSM3073802: G9a KO rep1 me3; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K9me3
Cell type
Cell type Class
Blood
Cell type
CD8+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
CD8+ T cells
strain
C57BL/6
tissue
spleen
cell type
T cell
antibody
H3K9me3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Native ChIP was performed. Nuclei from cultured CD8 T cells was extracted with 50mM Tris, 1mM CaCl2, 0.2% Triton, and proteinase inhibitors. Then nuclei were subjected to micrococcal nuclease digestion for 5min. Soluble chromatin was used for input and immunoprecipitation with anti-H3K9me3 (ab8898). DNA was prepped by Genome Technology Access Center using standard ChIP library prep for Illumina.