Native ChIP was performed. Nuclei from cultured CD8 T cells was extracted with 50mM Tris, 1mM CaCl2, 0.2% Triton, and proteinase inhibitors. Then nuclei were subjected to micrococcal nuclease digestion for 5min. Soluble chromatin was used for input and immunoprecipitation with anti-H3K9me3 (ab8898). DNA was prepped by Genome Technology Access Center using standard ChIP library prep for Illumina.