Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3T6ph

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
Prostate cancer cell line LNCaP
cell type
LNCaP
transfection
no treatment
passage
14-17
antibody
H3T6ph antibody (provided by Dr. Roland Schüle's lab)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
LNCaP and VCaP cells were harvested by scraping from the plates. Libraries were prepared according to Rubicon's instructions accompanying the ThruPLEX-FD kit (for ChIP-seq) or Illumina TruSeq RNA Sample Prep Kit v2 (for RNA-seq)

Sequencing Platform

instrument_model
Illumina HiSeq 1000

hg38

Number of total reads
22780662
Reads aligned (%)
78.9
Duplicates removed (%)
30.6
Number of peaks
578 (qval < 1E-05)

hg19

Number of total reads
22780662
Reads aligned (%)
78.3
Duplicates removed (%)
32.2
Number of peaks
519 (qval < 1E-05)

Base call quality data from DBCLS SRA