Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Embryo

Cell type

Embryonic face

NA

NA

Sample

source_name

facial mesenchyme

background strain

C57BL6/j

genotype/variation

wt

embryonic stage

11.5

chip antibody

anti-H3K27ac antibody (Abcam, catalog# ab4729, lot# GR7675-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from drbris of nuclei and DNA-wrapped histones were Immunoprecipitated with specific antibody. Libraries were prepared with NEB Next ChIP-seq Sample Prep Master Mix Set I (Cat.No.E6240S, NEB) and NEB Next Multiplex Oligos for Illumina (Cat.No: E7335S, NEB)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

19997495

Reads aligned (%)

98.9

Duplicates removed (%)

17.1

Number of peaks

8180 (qval < 1E-05)

mm9

Number of total reads

19997495

Reads aligned (%)

98.8

Duplicates removed (%)

17.2

Number of peaks

8196 (qval < 1E-05)