Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

lincRNA-p21+/+ murine MEF H3K27me3 ChIP-seq without Doxorubicin.

strain background

hybrid 129/B6

genotype/variation

wild type: lincRNA-p21+/+

cell type

mouse embryonic fibroblast (MEF)

treatment

Mock

chip antibody

H3K27me3

chip antibody vendor

abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and bound DNA fragments were isolated with antibody. Genomic DNA libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

14029891

Reads aligned (%)

98.5

Duplicates removed (%)

11.7

Number of peaks

162 (qval < 1E-05)

mm9

Number of total reads

14029891

Reads aligned (%)

98.4

Duplicates removed (%)

11.9

Number of peaks

166 (qval < 1E-05)