lincRNA-p21+/+ murine MEF H3K4me3 ChIP-seq with Doxorubicin
strain background
hybrid 129/B6
genotype/variation
wild type: lincRNA-p21+/+
cell type
mouse embryonic fibroblast (MEF)
treatment
0.5 mM doxorubicin for 24hrs
chip antibody
H3K4me3
chip antibody vendor
abcam
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and bound DNA fragments were isolated with antibody. Genomic DNA libraries were prepared for sequencing using standard Illumina protocols.