Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

CD8+ T cells

NA

NA

Sample

source_name

CD8+ T cells

genotype

CAR-

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Nuclei were extracted from sorted CD8+ T cells and transposed with Tn5 transposase (Illumina Nextera) as per Buenrostro et al. Nature Methods, 2013 (PMID: 24097267) Libraries were generated using a single PCR-based addition of Illumina compatible 5' and 3' ends with 8bp, dual indeces

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

98975732

Reads aligned (%)

95.1

Duplicates removed (%)

37.3

Number of peaks

57387 (qval < 1E-05)

hg19

Number of total reads

98975732

Reads aligned (%)

94.9

Duplicates removed (%)

37.5

Number of peaks

57181 (qval < 1E-05)