GSM3069709: AR Crispr 818PTEN ERG ARIP; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Prostate
Cell type
Prostate cancer
NA
NA
Attributes by original data submitter
Sample
source_name
Prostate cancer
strain
129/C57Bl6
genotype
Pten-/- Rosa26-Tmprss2-ERG
manipulation
CRISPR-AR
tissue
Prostate cancer
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
cells are fixed by 0.8%PFA and lysised in low-salt wash buufer with 0.1% SDS and 1% Triton X-100 CHIP-Seq was performed by New York genome center. DNA was adenylated, ligated to Illumina sequencing adapters, and amplified by PCR (using 10 cycles). Final libraries were quantified using the KAPA Library Quantification Kit (KAPA Biosystems), Next generation sequencing was carried out on an Illumina HiSeq2000 plateform with 50 bp or 100 bp single reads.