Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Pancreas
Cell type
EndoC-BH1
NA
NA

Attributes by original data submitter

Sample

source_name
EndoC βH1
cell line
EndoC BetaH1
cell type
Pancreatic Beta cells
chip antibody
anti-H3K27Ac(Abcam Cat. # ab4729, Lot # GR132150-4)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The chromatin immune-precipitation was performed with 50,000 EndoC-βH1 cells using True MicroChIP Kit Kit (Diagenode Cat. # C01010130). The sequencing library was generated using MicroPlex Library Preparation Kit v2 (Diagenode Cat. # C05010012) on an IP-Star® Compact Automated System (Diagenode Cat. # B03000002)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
30901779
Reads aligned (%)
97.3
Duplicates removed (%)
21.1
Number of peaks
5076 (qval < 1E-05)

hg19

Number of total reads
30901779
Reads aligned (%)
96.6
Duplicates removed (%)
21.9
Number of peaks
4832 (qval < 1E-05)

Base call quality data from DBCLS SRA