The hollow, muscular organ that maintains the circulation of the blood.
Attributes by original data submitter
Sample
source_name
P1 Heart Input
background strain
C57BL/6
tissue
whole heart
treatment
-
induction time
-
development stage
P1
antibody
Input (IgG control)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
MEFs two or seven days after retroviral transduction were crosslinked with 1% formaldehyde in PBS for 30 min and neutralized by the addition of glycine to a final concentration of 0.125M for 5min. MEFs were then harvested and washed with cold PBS for ChIP. For mouse heart sample preparation, ChIP was performed as previously described (Huang et al., 2016; Xu et al., 2012), or by using ChIP-IT Express ChIP kits (Active Motif) following the vender's protocol. ChIP-seq libraries were generated using KAPA Hyper Prep Kit following the manufacturer's protocol (Kapa Biosystems).