Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Neural

Cell type

T98G

Primary Tissue

Brain

Tissue Diagnosis

Glioblastoma

Sample

source_name

T98G

cell line

T98G

chip antibody

H3K27me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Antibodies used were – EZH2 (Abcam 186006), and H3K27me3 (Millipore 07-449). Libraries were prepared according to the New England Biolabs NEBNext library prep kit (E6240L). Ampure XP beads (A63881) were used for size-selection and clean-up steps.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

34856973

Reads aligned (%)

90.1

Duplicates removed (%)

23.3

Number of peaks

415 (qval < 1E-05)

hg19

Number of total reads

34856973

Reads aligned (%)

89.5

Duplicates removed (%)

23.4

Number of peaks

277 (qval < 1E-05)