Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Neural
Cell type
T98G
Primary Tissue
Brain
Tissue Diagnosis
Glioblastoma

Attributes by original data submitter

Sample

source_name
T98G
cell line
T98G
chip antibody
EZH2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Antibodies used were – EZH2 (Abcam 186006), and H3K27me3 (Millipore 07-449). Libraries were prepared according to the New England Biolabs NEBNext library prep kit (E6240L). Ampure XP beads (A63881) were used for size-selection and clean-up steps.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
24808354
Reads aligned (%)
98.8
Duplicates removed (%)
39.2
Number of peaks
594 (qval < 1E-05)

hg38

Number of total reads
24808354
Reads aligned (%)
100.2
Duplicates removed (%)
38.9
Number of peaks
718 (qval < 1E-05)

Base call quality data from DBCLS SRA