Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

Testis

strain

C57BL/6

tissue

Testis

antibody

Millipore (#07-473)

genotype

Prdm9 (K/K) (lacks KRAB domain)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was extracted from whole testis after sonication ChIP was performed on fixed tissue

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

146907695

Reads aligned (%)

96.7

Duplicates removed (%)

30.5

Number of peaks

56067 (qval < 1E-05)

mm9

Number of total reads

146907695

Reads aligned (%)

96.6

Duplicates removed (%)

30.5

Number of peaks

56196 (qval < 1E-05)