HeLa Tet-On cells were lysed, sonicated. The resulting whole cell extract was centrifuged. The supernatant was incubated with RAD21 or NIPBL antibody. RAD21 or NIPBL bound DNA was isolated and purified. Next-generation sequencing library preparation using KAPA Hyper Prep kit (KAPA Biosystems) using TruSeq adapters (Integrated DNA Technologies). A quality check of the purified libraries was carried out using the Tapestation 4200 (Agilent).