Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Bone

Cell type

Osteoclasts

MeSH Description

A large multinuclear cell associated with the BONE RESORPTION. An odontoclast, also called cementoclast, is cytomorphologically the same as an osteoclast and is involved in CEMENTUM resorption.

Sample

source_name

Osteoclast cells

cell type

Osteoclast

genotype

ASXL1 KO

strain

C57BL/6

chip antibody

H3K4me3 (abcam 8580), lot #GR273043-3)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

33152350

Reads aligned (%)

98.0

Duplicates removed (%)

23.4

Number of peaks

17537 (qval < 1E-05)

mm9

Number of total reads

33152350

Reads aligned (%)

97.5

Duplicates removed (%)

23.3

Number of peaks

17603 (qval < 1E-05)