Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Digestive tract

Cell type

RKO

Primary Tissue

Colon

Tissue Diagnosis

Carcinoma

Sample

source_name

RKO

cell line

RKO

cell type

colorectal cancer cells

treatment

DMSO_day4

antibody

Anti-Acetyl Histone H3 [Lys27] (#39159, Active Motif, Lot#12613005)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP samples were purified by QIAquick PCR purification kit (Qiagen, Hilden, Germany) and total RNAs were collected using RNeasy (Invitrogen, Carlsbad, CA) Libraries were prepared using NEBNext ChIP-Seq Library Preparation Set for Illumina (New England Biolabs) for ChIP-seq. TruSeq Stranded mRNA Sample Prep Kit (Illumina) were used for RNA-seq following the manufacturer's protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 1500

hg38

Number of total reads

17495051

Reads aligned (%)

96.0

Duplicates removed (%)

1.8

Number of peaks

3244 (qval < 1E-05)

hg19

Number of total reads

17495051

Reads aligned (%)

95.1

Duplicates removed (%)

2.8

Number of peaks

3181 (qval < 1E-05)