Grown on matrigel/MEF conditioned DMEM/F12, 20% KO serum
treatment
4h DMSO treated
chip antibody
normal rabbit IgG
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed for 10min with 1% Formaldehyde, nuclei were extracted (hypotonic lysis), DNA sheared by sonication Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).