Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Liver

Cell type

Hepatocytes

MeSH Description

The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.

Sample

source_name

WT_hepatocytes

strain background

C57Bl/6; 129Sv

genotype/variation

wild type

age

postnatal 10 weeks

cell type

hepatocytes

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Liver were perfused and hepatocytes were isolated and lysed. Lysates were clarified from sonicated nuclei and Yap-DNA complexes were isolated with antibody. Purified ChIP DNA was used to prepare sequencing libraries via NEBNext Ultra DNA Library Prep Kit for the Illumina (E7370).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

20143169

Reads aligned (%)

98.0

Duplicates removed (%)

9.9

Number of peaks

392 (qval < 1E-05)

mm9

Number of total reads

20143169

Reads aligned (%)

97.8

Duplicates removed (%)

10.0

Number of peaks

428 (qval < 1E-05)