Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Blood
Cell type
CD34+
NA
NA

Attributes by original data submitter

Sample

source_name
human FL CD34+CD38-/lowCD90+GFP+
cell type
4 wk cultured FL-HSPC
genotype/variation
control

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Chromatin was tagmented and DNA extracted (Nextera DNA Library Prep Kit, Illumina) and incubated at 37oC for 30 minutes, (see methods) Transposed DNA was column purified and used for library amplification with custom made adaptor primers using NEBNext High-Fidelity 2x PCR Master Mix (New England Labs)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
14051803
Reads aligned (%)
93.0
Duplicates removed (%)
69.2
Number of peaks
3423 (qval < 1E-05)

hg19

Number of total reads
14051803
Reads aligned (%)
92.8
Duplicates removed (%)
69.5
Number of peaks
3419 (qval < 1E-05)

Base call quality data from DBCLS SRA