Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Pancreas
Cell type
Pancreatic islets
NA
NA

Attributes by original data submitter

Sample

source_name
pancreatic islets, H3K36me3 ChIP
tissue
pancreatic islets
Sex
male
age
36
purity
85
viability
95
chip antibody
rabbit anti-H3K36me3 (Abcam ab9050; lots 446805, 572220)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Crosslinked chromatin was prepared and immunoprecipitated as described in Stitzel ML, et al. (Cell Metab, 2010). Libraries were prepared and sequenced using standard Illumina protocols for GAII.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg38

Number of total reads
7304016
Reads aligned (%)
97.8
Duplicates removed (%)
16.4
Number of peaks
218 (qval < 1E-05)

hg19

Number of total reads
7304016
Reads aligned (%)
97.2
Duplicates removed (%)
17.2
Number of peaks
296 (qval < 1E-05)

Base call quality data from DBCLS SRA