GSM3027429: H3K4me3 MLL-AF4 ChIPSeq Rep4; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
H3K4me3 MLL-AF4 ChIPSeq
cell type
human ESC
genotype/variation
MLL-AF4 expression
chip antibody
H3K4me3 (ab8580)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Formaldehyde crosslinked cell extracts were sonicated to 200-500 bp sized particles at 0.5-min intervals with Bioruptor (Diagenode) and the chromatin fraction was divided in three parts and incubated overnight with antibody Libraries were prepared using the kit: NEBNext Ultra DNA Library Prep kit for Illumina (Ref.:#7370) following the commercial protocol. Libraries QC: Bioanalyzer and quantification by qPCR using the kit: KAPA Library Quantification Kit (ref. KK4835, KapaBiosystems)