Antigen

Antigen Class

TFs and others

Antigen

Jarid2

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

embryonic stem cells

cell line

Mtf2 GT/GT

genotype

Gene trapping cassette disrupting Mtf CDS

antibody

Jarid2

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin extraction and ChIP were performed as described in Kloet, S. L. et al. (doi:10.1038/nsmb.3248). Five ng/sample of ChIP were prepared for sequencing with the Kapa Hyper-prep Kit (Kapa Biosystems) using NEXTflex adapters (Bio Scientific) and amplified with ten cycles of PCR. Libraries were size-selected to obtain 300bp fragments using E-gel (Invitrogen) and sequenced on an Illumina NextSeq machine to obtain 75bp reads.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

15207240

Reads aligned (%)

99.9

Duplicates removed (%)

0.0

Number of peaks

848 (qval < 1E-05)

mm9

Number of total reads

15207240

Reads aligned (%)

99.8

Duplicates removed (%)

0.0

Number of peaks

834 (qval < 1E-05)